Enhanced Sensitivity.
Dependable Detection.

Overcoming the Challenges of ROS Detection

The fluorescent probe DCFH-DA is commonly used in ROS detection methods.

However, it has several limitations, such as weak intracellular fluorescence, dye leakage, and photo-oxidation during observation.

Dojindo’s Total ROS Detection Kits overcome these issues with enhanced sensitivity and dependable results, making ROS analysis easier and more accurate with a probe based on the DCFH-DA principle, optimized for improved performance.

Promotion

ROS Detection Kits

PROMO 55%

From 15/01/2026 to 30/04/2026

Code Product Name Unit List Price (€) Net Price (€)
MT16-10 MitoBright ROS Deep Red – Mitochondrial Superoxide Detection 100 nmol x 1 364 164
MT16-12 MitoBright ROS Deep Red – Mitochondrial Superoxide Detection 100 nmol x 3 812 366
R252-10 ROS Assay Kit -Highly Sensitive DCFH-DA- 100 tests 660 298
R253-10 ROS Assay Kit -Photo-oxidation Resistant DCFH-DA- 100 tests 1004 452

ROS Assay Kit – Highly Sensitive DCFH-DA-

High sensitivity to ROS

Detection conditions
Ex. 488 nm / Em. 500 – 560 nm
Cell type:HeLa cells (scale bar: 50 μm)

Reactivity Compared with Existing DCFH-DA

ROS Assay Kit -Photo-oxidation Resistant DCFH-DA-

Resistance to Photo-oxidation

Reactivity Compared with Existing DCFH-DA

Comparison

Highly Sensitive
DCFH-DA		 Photo-oxidation
Resistant DCFH-DA		 Company T
Probe D		 Company T
Probe C
Unit 100 tests 100 tests 100 mg 250 μL
Sensitivity ••••• •••• ••• •••
Photo-oxidation Resistance – ••••• – –
Cell Fixation – ••••• – •••

ROS x Mitochondria:
Superoxide Detection

MitoBright ROS Deep Red Mitochondrial Superoxide Detection

MitoBright ROS Deep Red enables sensitive detection of mitochondrial superoxide in live cells with long-wavelength fluorescence (Ex/Em: 540/670 nm).

This probe offers high selectivity for superoxide and can be co-stained with commonly used green or red fluorescent probes, providing flexibility for observation across various experimental setups.

Comparison with the competitor’s product

Dojindo
MitoBright ROS Deep Red		 Company T’sw
Product Red
Storage 0-5°C –5°C to –25°C
Detection Live cells Live cells
Excitation/Emission 540/670 nm 510/580 nm
Compatible Machines FCM, Plate Reader Microscope FCM, Microscope
Fixation after staining N/A N/A

ROS Assay Kit – Highly Sensitive DCFH-DA-

(Blue) Nucleus: Hoechst33342 (Ex=405, Em=450-495 nm)
(Green) Mitochondrial Mass: MitoTrackerTM Green FM (Ex=488 nm, Em=500-550 nm)
(Red) Mitochondrial Membrane Protential: TMRE (Ex=561 nm, Em=560-620 nm)
(Deep Red) Mitochondrial Superoxide: MitoBright ROS Deep Red (Ex=633 nm, Em=640-700 nm
Scale bar: 10 μm

Fornitore