MGL FAST
Master Mix, Ready to Load

Extremely fast end-point PCR mix for robust and accurate target detection for single- and multiplex reactions:

  • PCR Results in 20 minutes
  • analyze one or multiple targets per reaction
  • load directly on gel after PCR

PCR

Catalog Number Description Pricese
24-02-00001 MGL-FAST Master Mix, Ready to Load
1 ml | 250 rxn
€116,80
24-02-00001-5 MGL-FAST Master Mix, Ready to Load
5 ml | 1250 rxn
€553,13
24-02-00020 MGL-FAST Master Mix, Ready to Load
20 ml | 5000 rxn
€2.114,24

MGL Script RT cDNA Synthesis Kit

Superior specificity with gene-specific primers in flexible kit format

Novel in silico-engineered, thermostable reverse transcriptase with reduced RNase H activity. MGL Script RT is extremely stable and will remain fully active for up to one month at room temperature.

  • superior specificity with gene-specific primers
  • wide reaction temperature 37°C – 60°C
  • reaction set-up and shipment without dry ice
  • in flexible 6-tube kit format

cDNA Synthesis

Catalog Number Description Pricese
06-35-00050 MGL Script RT cDNA synthesis KIT
50 rxn | 20 μ l rxn
€159,90
06-35-00200 MGL Script RT cDNA synthesis KIT
200 rxn | 20 μ l rxn
€609,40

MGL FIREScrip RT cDNA synthesis MIX

Convenient cDNA synthesis mix with fast and robust FIREScript Reverse Transcriptase

Genetically modified MMLV-based reverse transcriptase with increased thermostability and improved performance at elevated temperatures.

  • high specificity and yield
  • wide reaction temperature from 37°C to 60°C
  • fast 15 min reaction time
  • a convenient 3-vial kit with all reaction components
  • reaction set-up and shipment without ice

cDNA Synthesis

Catalog Number Description Pricese
06-20-00100 MGL FIREScript RT cDNA synthesis MIX
Oligo (dT) and Random Primers 100 rxn | 100 x 20 μl rxn
€ 290,80
06-35-00200 MGL FIREScript RT cDNA synthesis MIX
Oligo (dT) and Random Primers 200 rxn | 500 x 20 μl rxn
€ 1.373,50

MGL Script 1-step MGL Green Kit 2.0

Highly sensitive dye-based one-step RT-qPCR kit with improved detection of low-copy target

  • cDNA synthesis and dye-based qPCR in one reaction
  • Great PCR efficiency – reliable and easy-to-analyze results
  • High sensitivity – great when working with low concentrated samples
  • Excellent fluorescence levels
  • Convenient reaction set-up at room temperature
  • Environmentally friendly – worldwide ice-free shipping

RT-qPCR

Catalog Number Description Pricese
08-91-00250 MGL Script 1-step MGL Green Kit 2.0
250 rxn | 250 x 20 μ l rxn
€ 381,00

PERFORMANCE

MGL FAST / MGL Green qPCR Mix

Extremely fast and highly sensitive dye-based qPCR

Extremely fast and highly sensitive dye-based qPCR for most demanding applications.

  • qPCR results 2x faster
  • highly sensitive MGLGreen® dye
  • reaction set-up and shipment without dry ice

qPCR

Catalog Number Description Rox Pricese
28-46-00001 MGL FAST MGL Green qPCR Mix
1 ml | 250 rxn
€ 92,56
28-41-00001 MGL FAST MGL Green qPCR Mix
1 ml | 250 rxn
€ 92,56
28-46-00001-5 MGL FAST MGL Green qPCR Mix
5×1 ml | 1250 rxn
€ 578,50
28-41-00001-5 MGL FAST MGL Green qPCR Mix
5×1 ml | 1250 rxn
€ 92,56
28-46-00020 MGL FAST MGL Green qPCR Mix
20 ml | 5000 rxn
€ 2.198,80
28-41-00020 MGL FAST MGL Green qPCR Mix
20 ml | 5000 rxn
€ 2.336.08

ROUTINE

MGL HOT FIREPol MGL Green qPCR Supermix

Highly specific and reproducible dye-based qPCR Mix with blue visualization dye for easy pipetting

Optimized ready-to-use solution for dye-based real-time quantitative PCR assays. This master mix has been developed to give highly specific and sensitive results and demonstrates excellent performance with GC-rich regions.

  • high sensitivity with low DNA concentrations
  • blue dye to ease pipetting
  • reduced primer dimer formation
  • one qPCR mix for all cyclers (except capillary and high ROX* cyclers)
  • contains dUTP to prevent carryover contamination with UNG treatment

qPCR

Catalog Number Description Pricese
08-36-00001 MGL HOT FIREPol MGL Green qPCR Supermix
1 ml | 250 rxn
€ 104,12
08-36-00001-5 MGL HOT FIREPol MGL Green qPCR Supermix
5×1 ml | 1250 rxn
€ 520,62
08-36-00001-10 MGL HOT FIREPol MGL Green qPCR Supermix
10x1ml | 2500 rxn
€ 994,67
08-36-00020 MGL HOT FIREPol MGL Green qPCR Supermix
20 ml | 5000 rxn
€ 1.897,28

ROUTINE

MGL HOT FIREPol EvaGreen qPCR Mix Plus

Cost-effective dye-based qPCR Mix with passive reference dye

  • high sensitivity and specificity
  • excellent efficiency
  • reaction set-up and shipment without dry ice
  • cost-effective solution for a wide range of applications

qPCR

Catalog Number Description Rox Pricese
08-24-00001 MGL HOT FIREPol EvaGreen qPCR Mix Plus
1 ml | 250 rxn
€ 92,56
08-25-00001 MGL HOT FIREPol EvaGreen qPCR Mix Plus
1 ml | 250 rxn
€ 92,56
08-24-00001-5 MGL HOT FIREPol EvaGreen qPCR Mix Plus
5×1 ml | 1250 rxn
€ 462,79
08-25-00001-5 MGL HOT FIREPol EvaGreen qPCR Mix Plus
5×1 ml | 1250 rxn
€ 462,79
08-24-00001-10 MGL HOT FIREPol EvaGreen qPCR Mix Plus
10×1 ml | 2500 rxn
€ 879,54
08-25-00001-10 MGL HOT FIREPol EvaGreen qPCR Mix Plus
10×1 ml | 2500 rxn
€ 879,54
08-24-00001-10 MGL HOT FIREPol EvaGreen qPCR Mix Plus
20 ml | 5000 rxn
€ 1.688,90
08-25-00001-10 MGL HOT FIREPol EvaGreen qPCR Mix Plus
20 ml | 5000 rxn
€ 1.688,90

HOT FIREPol Blend Master Mix with 12.5 mM MgCl2 (cod. 04-27-00125)

MGLFAST® Master Mix Ready to Load (cod. 24-02-00001)

Risultati dei test sulle due mastermix:

  • MGL HOT FIREPol® Blend Master Mix with 12.5 mM MgCl2 – Size: 0,1ml (25 rxn)
  • MGLFAST® Master Mix, Ready to Load – Size: 0,2ml (50 rxn)

Entrambe le mix hanno performato bene su campioni di matrice eterogenea, estratti sia da kit commerciali che tramite step in NaOH (idrossido di sodio 0,1 M).

La master mix MGL Hot Fire (con attività di proofreading) risulta essere ottimale per campioni destinati al sequenziamento.

La master mix MGLFast è ottima per analisi PCR-EndPoint.

Dr. Agnese Saporiti

FEM2 – Ambiente (Food, Environment, ManageMent)
fem2ambiente.com

ADDIITONAL REAGENTS – DNA LADDER

MGL 100 bp DNA Ladder Ready to Load

13 discrete DNA fragments from 100 bp to 3000 bp

The 100 bp DNA Ladder is a ready-to-load molecular weight marker suitable for DNA fragment size determination on gel electrophoresis

  • thirteen crisp bands
  • stable at room temperature
  • tracking dye included

DNA Ladder

Catalog Number Description Pricese
07-11-00050 MGL 100 bp DNA Ladder Ready to Load
50 μg | 500 μ l
€ 60,66
07-11-00050-5 MGL 100 bp DNA Ladder Ready to Load
250 μg (5 x 50 μg) | 2,5 ml
€ 287,06
07-11-00050-10 MGL 100 bp DNA Ladder Ready to Load
500 μg (10 x 50 μg) | 5 ml
€ 574,06

ADDIITONAL REAGENTS – DNA LADDER

MGL 1 kb DNA Ladder Ready to Load

13 discrete DNA fragments ranging from 250 bp to 10000 bp

The 1 kb DNA Ladder is a ready-to-use molecular weight marker suitable for DNA fragment size determination on gel electrophoresis

  • thirteen crisp bands
  • stable at room temperature
  • two tracking dyes

DNA Ladder

Catalog Number Description Pricese
07-12-00050 MGL 1 kb DNA Ladder Ready to Load
50 μg | 500 μ l
€ 45,66
07-12-00050-10 MGL 1 kb DNA Ladder Ready to Load
10 x 50 μg | 500 μ l
A RICHIESTA

BUFFERS

MGL RiboGrip® RNase Inhibitor (220U/μl)

Novel in silico engineered, proteinbased RNase inhibitor

  • Exceptional stability – stable for up to 60 °C for 1 hour
  • Unique high concentration formulation
  • Efficient protection of RNA at low DTT concentrations

DNA Ladder

Catalog Number Description Pricese
06-26-4000U MGL RiboGrip® RNase Inhibitor (220U/μl)
4000 U | 18 μl
€ 60,66
06-26-010kU MGL RiboGrip® RNase Inhibitor (220U/μl)
10000 U | 45 μl
€ 260,06

BUFFERS

MGL Salini UNG® Uracil-N-Glycosylase

Fast and robust heat-labile Uracil-NGlycosylase

  • Stable at 25 °C for at least 3 months and at 37 °C 2 weeks
  • Heat-labile enzyme (compatible with Sanger sequencing). No reactivation is detected after heat inactivation.
  • Fast 30 second reaction time
  • Tolerant to common inhibitors
  • Reaction set-up and shipment without ice
  • Glycerol-free formulation is availableice

DNA Ladder

Catalog Number Description Pricese
31-01-00100 MGL Salini UNG® Uracil-N-Glycosylase
100 U | 100 μl
€ 82,10
31-01-00100-5 MGL Salini UNG® Uracil-N-Glycosylase
500 U (5 x 100 U) | 5 x 100 μl
€ 410,50