MGL FAST
Master Mix, Ready to Load
Extremely fast end-point PCR mix for robust and accurate target detection for single- and multiplex reactions:
- PCR Results in 20 minutes
- analyze one or multiple targets per reaction
- load directly on gel after PCR
PCR
Catalog Number | Description | Pricese |
24-02-00001 | MGL-FAST Master Mix, Ready to Load 1 ml | 250 rxn |
€116,80 |
24-02-00001-5 | MGL-FAST Master Mix, Ready to Load 5 ml | 1250 rxn |
€553,13 |
24-02-00020 | MGL-FAST Master Mix, Ready to Load 20 ml | 5000 rxn |
€2.114,24 |
MGL Script RT cDNA Synthesis Kit
Superior specificity with gene-specific primers in flexible kit format
Novel in silico-engineered, thermostable reverse transcriptase with reduced RNase H activity. MGL Script RT is extremely stable and will remain fully active for up to one month at room temperature.
- superior specificity with gene-specific primers
- wide reaction temperature 37°C – 60°C
- reaction set-up and shipment without dry ice
- in flexible 6-tube kit format
cDNA Synthesis
Catalog Number | Description | Pricese |
06-35-00050 | MGL Script RT cDNA synthesis KIT 50 rxn | 20 μ l rxn |
€159,90 |
06-35-00200 | MGL Script RT cDNA synthesis KIT 200 rxn | 20 μ l rxn |
€609,40 |
MGL FIREScrip RT cDNA synthesis MIX
Convenient cDNA synthesis mix with fast and robust FIREScript Reverse Transcriptase
Genetically modified MMLV-based reverse transcriptase with increased thermostability and improved performance at elevated temperatures.
- high specificity and yield
- wide reaction temperature from 37°C to 60°C
- fast 15 min reaction time
- a convenient 3-vial kit with all reaction components
- reaction set-up and shipment without ice
cDNA Synthesis
Catalog Number | Description | Pricese |
06-20-00100 | MGL FIREScript RT cDNA synthesis MIX Oligo (dT) and Random Primers 100 rxn | 100 x 20 μl rxn |
€ 290,80 |
06-35-00200 | MGL FIREScript RT cDNA synthesis MIX Oligo (dT) and Random Primers 200 rxn | 500 x 20 μl rxn |
€ 1.373,50 |
MGL Script 1-step MGL Green Kit 2.0
Highly sensitive dye-based one-step RT-qPCR kit with improved detection of low-copy target
- cDNA synthesis and dye-based qPCR in one reaction
- Great PCR efficiency – reliable and easy-to-analyze results
- High sensitivity – great when working with low concentrated samples
- Excellent fluorescence levels
- Convenient reaction set-up at room temperature
- Environmentally friendly – worldwide ice-free shipping
RT-qPCR
Catalog Number | Description | Pricese |
08-91-00250 | MGL Script 1-step MGL Green Kit 2.0 250 rxn | 250 x 20 μ l rxn |
€ 381,00 |
PERFORMANCE
MGL FAST / MGL Green qPCR Mix
Extremely fast and highly sensitive dye-based qPCR
Extremely fast and highly sensitive dye-based qPCR for most demanding applications.
- qPCR results 2x faster
- highly sensitive MGLGreen® dye
- reaction set-up and shipment without dry ice
qPCR
Catalog Number | Description | Rox | Pricese |
28-46-00001 | MGL FAST MGL Green qPCR Mix 1 ml | 250 rxn |
€ 92,56 | |
28-41-00001 | MGL FAST MGL Green qPCR Mix 1 ml | 250 rxn |
€ 92,56 | |
28-46-00001-5 | MGL FAST MGL Green qPCR Mix 5×1 ml | 1250 rxn |
€ 578,50 | |
28-41-00001-5 | MGL FAST MGL Green qPCR Mix 5×1 ml | 1250 rxn |
€ 92,56 | |
28-46-00020 | MGL FAST MGL Green qPCR Mix 20 ml | 5000 rxn |
€ 2.198,80 | |
28-41-00020 | MGL FAST MGL Green qPCR Mix 20 ml | 5000 rxn |
€ 2.336.08 |
ROUTINE
MGL HOT FIREPol MGL Green qPCR Supermix
Highly specific and reproducible dye-based qPCR Mix with blue visualization dye for easy pipetting
Optimized ready-to-use solution for dye-based real-time quantitative PCR assays. This master mix has been developed to give highly specific and sensitive results and demonstrates excellent performance with GC-rich regions.
- high sensitivity with low DNA concentrations
- blue dye to ease pipetting
- reduced primer dimer formation
- one qPCR mix for all cyclers (except capillary and high ROX* cyclers)
- contains dUTP to prevent carryover contamination with UNG treatment
qPCR
Catalog Number | Description | Pricese |
08-36-00001 | MGL HOT FIREPol MGL Green qPCR Supermix 1 ml | 250 rxn |
€ 104,12 |
08-36-00001-5 | MGL HOT FIREPol MGL Green qPCR Supermix 5×1 ml | 1250 rxn |
€ 520,62 |
08-36-00001-10 | MGL HOT FIREPol MGL Green qPCR Supermix 10x1ml | 2500 rxn |
€ 994,67 |
08-36-00020 | MGL HOT FIREPol MGL Green qPCR Supermix 20 ml | 5000 rxn |
€ 1.897,28 |
ROUTINE
MGL HOT FIREPol EvaGreen qPCR Mix Plus
Cost-effective dye-based qPCR Mix with passive reference dye
- high sensitivity and specificity
- excellent efficiency
- reaction set-up and shipment without dry ice
- cost-effective solution for a wide range of applications
qPCR
Catalog Number | Description | Rox | Pricese |
08-24-00001 | MGL HOT FIREPol EvaGreen qPCR Mix Plus 1 ml | 250 rxn |
€ 92,56 | |
08-25-00001 | MGL HOT FIREPol EvaGreen qPCR Mix Plus 1 ml | 250 rxn |
€ 92,56 | |
08-24-00001-5 | MGL HOT FIREPol EvaGreen qPCR Mix Plus 5×1 ml | 1250 rxn |
€ 462,79 | |
08-25-00001-5 | MGL HOT FIREPol EvaGreen qPCR Mix Plus 5×1 ml | 1250 rxn |
€ 462,79 | |
08-24-00001-10 | MGL HOT FIREPol EvaGreen qPCR Mix Plus 10×1 ml | 2500 rxn |
€ 879,54 | |
08-25-00001-10 | MGL HOT FIREPol EvaGreen qPCR Mix Plus 10×1 ml | 2500 rxn |
€ 879,54 | |
08-24-00001-10 | MGL HOT FIREPol EvaGreen qPCR Mix Plus 20 ml | 5000 rxn |
€ 1.688,90 | |
08-25-00001-10 | MGL HOT FIREPol EvaGreen qPCR Mix Plus 20 ml | 5000 rxn |
€ 1.688,90 |
HOT FIREPol Blend Master Mix with 12.5 mM MgCl2 (cod. 04-27-00125)
MGLFAST® Master Mix Ready to Load (cod. 24-02-00001)
Risultati dei test sulle due mastermix:
- MGL HOT FIREPol® Blend Master Mix with 12.5 mM MgCl2 – Size: 0,1ml (25 rxn)
- MGLFAST® Master Mix, Ready to Load – Size: 0,2ml (50 rxn)
Entrambe le mix hanno performato bene su campioni di matrice eterogenea, estratti sia da kit commerciali che tramite step in NaOH (idrossido di sodio 0,1 M).
La master mix MGL Hot Fire (con attività di proofreading) risulta essere ottimale per campioni destinati al sequenziamento.
La master mix MGLFast è ottima per analisi PCR-EndPoint.
Dr. Agnese Saporiti
FEM2 – Ambiente (Food, Environment, ManageMent)
fem2ambiente.com
ADDIITONAL REAGENTS – DNA LADDER
MGL 100 bp DNA Ladder Ready to Load
13 discrete DNA fragments from 100 bp to 3000 bp
The 100 bp DNA Ladder is a ready-to-load molecular weight marker suitable for DNA fragment size determination on gel electrophoresis
- thirteen crisp bands
- stable at room temperature
- tracking dye included
DNA Ladder
Catalog Number | Description | Pricese |
07-11-00050 | MGL 100 bp DNA Ladder Ready to Load 50 μg | 500 μ l |
€ 60,66 |
07-11-00050-5 | MGL 100 bp DNA Ladder Ready to Load 250 μg (5 x 50 μg) | 2,5 ml |
€ 287,06 |
07-11-00050-10 | MGL 100 bp DNA Ladder Ready to Load 500 μg (10 x 50 μg) | 5 ml |
€ 574,06 |
ADDIITONAL REAGENTS – DNA LADDER
MGL 1 kb DNA Ladder Ready to Load
13 discrete DNA fragments ranging from 250 bp to 10000 bp
The 1 kb DNA Ladder is a ready-to-use molecular weight marker suitable for DNA fragment size determination on gel electrophoresis
- thirteen crisp bands
- stable at room temperature
- two tracking dyes
DNA Ladder
Catalog Number | Description | Pricese |
07-12-00050 | MGL 1 kb DNA Ladder Ready to Load 50 μg | 500 μ l |
€ 45,66 |
07-12-00050-10 | MGL 1 kb DNA Ladder Ready to Load 10 x 50 μg | 500 μ l |
A RICHIESTA |
BUFFERS
MGL RiboGrip® RNase Inhibitor (220U/μl)
Novel in silico engineered, proteinbased RNase inhibitor
- Exceptional stability – stable for up to 60 °C for 1 hour
- Unique high concentration formulation
- Efficient protection of RNA at low DTT concentrations
DNA Ladder
Catalog Number | Description | Pricese |
06-26-4000U | MGL RiboGrip® RNase Inhibitor (220U/μl) 4000 U | 18 μl |
€ 60,66 |
06-26-010kU | MGL RiboGrip® RNase Inhibitor (220U/μl) 10000 U | 45 μl |
€ 260,06 |
BUFFERS
MGL Salini UNG® Uracil-N-Glycosylase
Fast and robust heat-labile Uracil-NGlycosylase
- Stable at 25 °C for at least 3 months and at 37 °C 2 weeks
- Heat-labile enzyme (compatible with Sanger sequencing). No reactivation is detected after heat inactivation.
- Fast 30 second reaction time
- Tolerant to common inhibitors
- Reaction set-up and shipment without ice
- Glycerol-free formulation is availableice
DNA Ladder
Catalog Number | Description | Pricese |
31-01-00100 | MGL Salini UNG® Uracil-N-Glycosylase 100 U | 100 μl |
€ 82,10 |
31-01-00100-5 | MGL Salini UNG® Uracil-N-Glycosylase 500 U (5 x 100 U) | 5 x 100 μl |
€ 410,50 |