Apoptosis Detection Kits

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Cell Apoptosis

Apoptosis: A Key Process in Programmed Cell Death, a form of programmed cell death (PCD), is a genetically controlled and orderla form of programmed cell death (PCD), is a genetically regulated process essential for cell growth, development, and renewal. Key events include phosphatidylserine (PS) externalization, mitochondrial membrane potential (ΔΨm) changes, caspase activation, and DNA fragmentation.

This orderly self-destruction occurs under physiological or pathological conditions, helping organisms adapt to their environment. Proper apoptosis is critical for maintaining cellular balance, while dysregulation can lead to diseases such as cancer and neurodegenerative disorders.

Elabscience® Apoptosis Detection Kits

Apoptosis detection is a critical tool for analyzing cell fate and disease mechanisms. Elabscience® offers comprehensive apoptosis detection kits covering multiple key apoptotic pathways, enabling precise identification of essential steps in the cell death process.

Annexin V Assay Kits

  • 15 fluorochromes paired with 3 nuclear dyes
  • Complete the experiment in just 15 minutes
  • Distinct cell populations, effective even with high cell density

TUNEL Assay Kits

  • Free of dimethyl arsenic, safe and non-toxic
  • Wide variety, compatible with all detection equipment
  • High sensitivity, comparable to leading international brands

Caspase Assay Kits

  • Comprehensive panel, detects 8 types of Caspases
  • Wide variety, available in both fluorescence and spectrophotometry methods
  • Simple operation, delivering accurate experimental results

Mitochondrial Assay Kits

  • Early apoptosis detection
  • Simple experimental procedure
  • Mitochondrial localization and function assessment

Apoptopic process events

  1. The cell membrane loses its symmetry
  2. Activation of apoptosis-related proteins such as Bcl-2
  3. Caspase Activation
  4. Mitochondrial membrane potential collapses, cytometers C is released
  5. Increase in sub-G1 cell population
  6. Cell nuclear shrinkage
  7. DNA fragmentation
  8. Cell membrane budding to form apoptotic bodies

Annexin V Assay Kits and Related Reagents

PI

Fluorochrome Product Name
FITC Annexin V-FITC/PI Apoptosis Kit
Elab Fluor® 647 Annexin V-Elab Fluor® 647/PI Apoptosis Kit
APC Annexin V-APC/PI Apoptosis Kit
EGFP Annexin V-EGFP/PI Apoptosis Kit
Cyanine5 Annexin V-Cyanine5/PI Apoptosis Kit
APC/Cyanine7 Annexin V-APC/Cyanine7/PI Apoptosis Kit
Elab Fluor® Violet 450 Annexin V-Elab Fluor® Violet 450/PI Apoptosis Kit
Elab Fluor® Violet 500 Annexin V-Elab Fluor® Violet 500/PI Apoptosis Kit
Elab Fluor® 488 Annexin V-Elab Fluor® 488/PI Apoptosis Kit
Elab Fluor® Red 780 Annexin V-Elab Fluor® Red 780/PI Apoptosis Kit

7-AAD

Fluorochrome Product Name
FITC Annexin V-FITC/7-AAD Apoptosis Kit
Elab Fluor® 647 Annexin V-Elab Fluor® 647/7-AAD Apoptosis Kit
PE Annexin V-PE/7-AAD Apoptosis Kit
APC Annexin V-APC/7-AAD Apoptosis Kit
EGFP Annexin V-EGFP/7-AAD Apoptosis Kit
Cyanine5 Annexin V-Cyanine5/7-AAD Apoptosis Kit
APC/Cyanine7 Annexin V-PE/Cyanine7/7-AAD Apoptosis Kit
Elab Fluor® Violet 450 Annexin V-Elab Fluor® Violet 450/7-AAD Apoptosis Kit
Elab Fluor® Violet 500 Annexin V-Elab Fluor® Violet 500/7-AAD Apoptosis Kit
Elab Fluor® 488 Annexin V-Elab Fluor® 488/7-AAD Apoptosis Kit
Elab Fluor® Red 780 Annexin V-Elab Fluor® Red 780/7-AAD Apoptosis Kit

DAPI

Fluorochrome Product Name
FITC Annexin V-FITC/DAPI Apoptosis Kit
Elab Fluor® 647 Annexin V-Elab Fluor® 647/DAPI Apoptosis Kit
PE Annexin V-PE/DAPI Apoptosis Kit
APC Annexin V-APC/DAPI Apoptosis Kit
EGFP Annexin V-EGFP/DAPI Apoptosis Kit
Cyanine5 Annexin V-Cyanine5/DAPI Apoptosis Kit
PE/Cyanine5 Annexin V-PE/Cyanine5/DAPI Apoptosis Kit
PE/Cyanine5.5 Annexin V-PE/Cyanine5.5/DAPI Apoptosis Kit
PE/Cyanine7 Annexin V-PE/Cyanine7/DAPI Apoptosis Kit
APC/Cyanine7 Annexin V-APC/Cyanine7/DAPI Apoptosis Kit
PE/Elab Fluor® 594 Annexin V-PE/Elab Fluor® 594/DAPI Apoptosis Kit
Elab Fluor® 488 Annexin V-Elab Fluor® 488/DAPI Apoptosis Kit
Elab Fluor® Red 780 Annexin V-Elab Fluor® Red 780/DAPI Apoptosis Kit

Related Reagents

Product Name
Recombinant Human ANXA5/Annexin V/Annexin A5 Protein (His tag)
ANXA5 Polyclonal Antibody
Annexin V Reagent
Annexin V Binding Buffer (10×)
Annexin V Lyophilized Powder

TUNEL Assay Kits

An important feature of the late stage of apoptosis is DNA fragmentation, and the exposed 3’-OH of the fragmented DNA can be catalyzed by terminal deoxynucleotidyl transferase (TdT) to bind to fluorochrome-labeled dUTP, which can be visualized by fluorescence microscopy or flow cytometry. This method is also known as One-step method (higher detection sensitivity, more intuitive results, and easier experimental results to be published in SCI articles).
If the exposed 3’-OH groups are labeled with biotin-labeled dUTP (Biotin-dUTP) under the catalysis of TdT, and then combined with HRP-labeled Streptavidin (Streptavidin-HRP), the result can be visualized by DAB color development under the catalysis of HRP, apoptotic cells can then be detected by an ordinary optical microscope.
Elabscience® developed the TUNEL series of apoptosis kits, including the One-step TUNEL In Situ Apoptosis Kit, One-step TUNEL Flow Cytometry Apoptosis Kit, and TUNEL In Situ Apoptosis Kit (HRP-DAB Method). These kits can be used for apoptosis detection in tissue samples (paraffin-embedded, frozen sections) and cell samples (cell smears, cell culture films, suspension cells, adherent cells). Our TUNEL assay kits have high sensitivity, quick and easy operation, and can better assist in apoptosis research related to cell function and the R&D of related drugs.

One-step TUNEL In Situ Apoptosis Kits

Elabscience® One-step TUNEL In Situ Apoptosis Kits are suitable for in situ apoptosis detection of tissue samples (paraffin-embedded, frozen sections) and cell samples (cell smears, slide films), and the detection results can be directly observed by fluorescence microscopy. Compared with the DAB colorimetric method, the experimental results of fluorescent TUNEL are fluorescent images, which are more intuitive and easier to publish in SCI articles.

Features of Elabscience® One-step TUNEL
In Situ Apoptosis Kits

Multiple Options
A variety of fluorescent dyes (FITC/ Elab Fluor® 488/ Elab Fluor® 594/ Elab Fluor® 647/ Elab Fluor® 555) kits are available.
Safe and Non-toxic
No dimethyl arsenate, non-toxic and harmless to people and the environment
Wide Range of Applications
Suitable for a variety of tissue samples (paraffin sections, frozen sections) and cell samples (cell slides)
Easy to Operate
One-step dyeing, saving time and effort.
Cost-effective
The key components are self-developed with more stable quality, and more cost-effective price

Elabscience® One-step TUNEL In Situ Apoptosis Kits

Product Name Dye Excitation/Emission Wavelength Size
One-step TUNEL In Situ Apoptosis Kit (Green, FITC) FITC, DAPI FITC: 495/525nm
DAPI: 350/470nm		 20/50/100 Assays
One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488) Elab Fluor® 488, DAPI Elab Fluor® 488: 495/519nm
DAPI: 350/470nm		 20/50/100 Assays
One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 594) Elab Fluor® 594, DAPI Elab Fluor® 594: 590/617nm
DAPI: 350/470nm		 20/50/100 Assays
One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 647) Elab Fluor® 647, DAPI Elab Fluor® 647: 650/665nm
DAPI: 350/470nm		 20/50/100 Assays
One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 555) Elab Fluor® 555, DAPI Elab Fluor® 555: 555/565nm
DAPI: 350/470nm		 20/100 Assays

Comparative Results

Comparison and display of green fluorescence results between R company and Elabscience® Illumination value: 10, exposure time: 100 ms

One-step TUNEL Flow Cytometry Apoptosis Kits

Elabscience® One-step TUNEL Flow Cytometry Apoptosis Kit is a highly sensitive and quick and easy detection product for apoptosis. This kit is suitable for apoptosis detection of suspension cells and adherent cells, and the detection results can be analyzed by flow cytometry.

Features of Elabscience® One-step TUNEL
Flow Cytometry Apoptosis Kits

Better detection scheme
Optimized fluorescent labeling solution, more suitable for flow cytometry detection schemes.
Easy to Operate
One-step dyeing, saving time and effort
Wide Range of Applications
Suitable for a variety of suspension cells and adherent cultured cells.
Multiple Options
FITC/Elab Fluor® 488/Elab Fluor® 594/Elab Fluor® Violet 450/Elab Fluor® 647/Elab Fluor® 555, a variety of fluorescence channels are available
Cost-effective
The kit contains fixed permeabilization fluid, no need to purchase additional reagents

Elabscience® One-step
TUNEL Flow Cytometry Apoptosis Kit

Product Name Fluorochromes Excitation/Emission Wavelength Size
One-step TUNEL Flow Cytometry Apoptosis Kit (Green, FITC) FITC 495/525nm 20/100 Assays
One-step TUNEL Flow Cytometry Apoptosis Kit (Green, Elab Fluor® 488) Elab Fluor® 488 495/519nm 20/100 Assays
One-step TUNEL Flow Cytometry Apoptosis Kit (Red, Elab Fluor® 594) Elab Fluor® 594 590/617nm 20/100 Assays
One-step TUNEL Flow Cytometry Apoptosis Kit (Blue, Elab Fluor® Violet 450) Elab Fluor® Violet 450 410/450nm 20/100 Assays
One-step TUNEL Flow Cytometry Apoptosis Kit (Red, Elab Fluor® 647) Elab Fluor® 647 650/665nm 20/100 Assays
One-step TUNEL Flow Cytometry Apoptosis Kit (Red, Elab Fluor® 555) Elab Fluor® 555 555/565nm 20/100 Assays

Comparative Results

Biological control
Jurkat cells cultured without camptothecin, labeled with working solution, and TdT enzyme added
Negative control
Jurkat cells cultured with 5μM camptothecin for 4h, labeled with working solution, without TdT enzyme
Experimental group
Jurkat cells cultured with 5 μM camptothecin for 4h, labeled with working solution, and TdT enzyme added

TUNEL In Situ Apoptosis Kit (HRP-DAB Method)

Elabscience® TUNEL In Situ Apoptosis Kit (HRP-DAB Method) is a highly sensitive product that can detect apoptosis quickly and easily. This kit is suitable for tissue samples (paraffin sections, frozen sections). After biotin labeling and subsequent DAB color development, apoptosis can be observed with an ordinary optical microscope.

Features of Elabscience® TUNEL
In Situ Apoptosis Kit (HRP-DAB Method)

High sensitivity
Very low background staining, strong positive staining and apoptosis can be detected at the single-cell level
Quick and easy
It only takes about 2~3 h to complete the staining
Wide range of applications
Suitable for a variety of tissue samples (paraffin sections, frozen sections)
Good specificity
It is generally easier to label apoptotic cells than necrotic cells in TUNEL assays
Safe and non-toxic
No dimethyl arsenate, non-toxic and harmless to people and the environment

Elabscience® TUNEL In Situ Apoptosis Kit
(HRP-DAB Method)

Cat.No. Product Name Size
E-CK-A331 TUNEL In Situ Apoptosis Kit (HRP-DAB Method) 20/50/100 Assays

Results of TUNEL In Situ Apoptosis Kit
(HRP-DAB Method)

Mouse Liver Tissue
Incubate with Dnase I for 10 min
Mouse Colon Tissue
Incubate with Dnase I for 10 min

Caspase

Caspase, cysteinyl aspartate-specific proteinase, is a group of proteases present in the cytoplasm with similar structures. They exhibit high homology and structural similarity, capable of specifically cleaving peptide bonds at aspartate residues in target proteins. Caspases are closely associated with apoptosis (pyroptosis) in eukaryotic cells and participate in the regulation of cell growth, differentiation, and apoptosis.
Elabscience®Caspase assay kits, for apoptosis/pyroptosis detection, high specificity, high sensitivity, simple and fast operation, excellent quality,can meet a wide range of your needs.

Detection

Colorimetric Method

Principle of Caspase Assay Kit (Colorimetric Method)

Caspase-specific peptide enzymes are linked to the yellow chromophore pNA (p-nitroanilide) as a substrate.Upon activation of Caspase, the enzyme cleaves the substrate, releasing the yellow chromophore. The absorbance of the released pNA can be measured using a spectrophotometer or enzyme reader at wavelengths around 405 nm or 400 nm, as pNA exhibits strong absorbance in this range.

Advantages

Multiple Choices
Provide a variety of Caspase assay kits to meet the different needs of customers
Cost-effective
The key components are developed in-house with reliable quality and more cost-effective price
Simple Operation
The composition of the reagent was optimized and the experimental operation was more time saving
Wide Applications
The enzyme activity in samples can be detected quantitatively, and these kits is suitable for a variety of cells

Caspase Assay Kits Colorimetric Method

Product Name Size Application
Caspase 1 Activity Assay Kit (Colorimetric Method) 20/100 Assays Cell Pyroptosis
Caspase 2 Activity Assay Kit (Colorimetric Method) 20/100 Assays Apoptosis Detection
Caspase 3/7 Activity Assay Kit (Colorimetric Method) 20/100 Assays Apoptosis Detection
Caspase 4 Activity Assay Kit (Colorimetric Method) 20/100 Assays Cell Pyroptosis
Caspase 6 Activity Assay Kit (Colorimetric Method) 20/100 Assays Apoptosis Detection
Caspase 8 Activity Assay Kit (Colorimetric Method) 20/100 Assays Apoptosis Detection
Caspase 9 Activity Assay Kit (Colorimetric Method) 20/100 Assays Apoptosis Detection

Fluorescence Method

Principle of Caspase Assay Kit (Fluorescence Method)

Caspase-specific peptides are conjugated to high-affinity DNA fluorochrome as substrates. These substrates can penetrate the cell membrane and enter the cytoplasm. The substrates themselves are non-fluorescent and exhibit charge repulsion with DNA. Upon activation of Caspase, the enzyme cleaves the substrate, releasing the high-affinity DNA fluorochrome. This fluorochrome binds to DNA, resulting in strong fluorescence. This method allows for the detection of Caspase activity and observation of nuclear morphological changes during the process of apoptosis.

Advantages

Easy to operate
One-step dyeing, saving time and effort
Live-cell observation
Without lysis of the cells, live cell observations can be performed
Safe and non-toxic
Low cytotoxicity and did not affect the apoptotic process of cells
Wide Applications
Can be combined with multiple assay kits, choose diversification

Caspase Assay Kits (Fluorescence Method)

Product Name Cat.No. Size
Caspase 1 Activity Detection Substrate for Flow Cytometry E-CK-A481 20/100 Tests
Caspase 3/7 Activity Detection Substrate for Flow Cytometry E-CK-A483 20/100 Tests
Caspase 4 Activity Detection Substrate for Flow Cytometry E-CK-A484 20/100 Tests
Caspase 6 Activity Detection Substrate for Flow Cytometry E-CK-A486 20/100 Tests
Caspase 8 Activity Detection Substrate for Flow Cytometry E-CK-A488 20/100 Tests
Caspase 9 Activity Detection Substrate for Flow Cytometry E-CK-A489 20/100 Tests
Caspase 3/7 and Annexin V Double Staining Apoptosis Kit E-CK-A831 20/100 Assays
Caspase 3/7 and PI Double Staining Kit E-CK-A832 20/100 Assays
Caspase 3/7 and DAPI Double Staining Kit E-CK-A833 20/100 Assays
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