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In vitro cell culture requires the long-term preservation of biological cell activity.
To achieve this, cells must be cryopreserved and revived when needed.
Currently, the most commonly used method for cell cryopreservation is storage in liquid nitrogen.
This process involves the addition of appropriate cryoprotective agents and the gradual cooling of cells to a specified temperature range, thereby protecting their viability.
The Serum-Free Cell Freezing Medium, developed by Pricella, is a specialized cryopreservation solution designed for a wide range of cell types. It has been optimized through extensive experimentation to support both cryopreservation and resuscitation in long-term cell research.
The formulation includes a cell sedimentation stabilizer, which slows the rate of cell settling during cryopreservation. This helps prevent cellular compression, which can compromise viability. In addition, the medium contains a blend of cryoprotectants—including membrane stabilizers, permeable intracellular protectants, and non-permeable extracellular agents.
These components interact with water molecules in the medium to reduce ice crystal formation by increasing viscosity and minimizing water crystallization. This significantly reduces ice-induced cell damage and enhances post-thaw survival rates.
The medium is serum-free and does not contain any animal-derived proteins, minimizing the risk of contamination from bacteria, viruses, or mycoplasma and ensuring the safety of preserved cells. It is suitable for a broad spectrum of cells, including conventional cell lines, primary cells, serum-free culture cells, and protein-expressing cells.
Compared to traditional cryopreservation media, this product eliminates the need for complex, time-consuming gradient freezing protocols or expensive programmable cooling devices. Cells can be directly resuspended in the medium, stored at −80°C overnight, and then transferred to liquid nitrogen the following day—streamlining the process and saving significant time and resources.
| Items | Size | List Price | Promo |
| PB180438 | 100mL | $ 118.00 | 82,60 € |
| PB180438 | 50mL | $ 65.00 | 45,50 € |
| PB180438 | 10mL×5 | $ 68.00 | 47,60 € |
| Brand | Product name | SKU | Component | Pack size | Price | Storage |
| Stemcell | CryoStor®CS2 | 07932 | 2% DMSO, serum-free, animal-derived proteins | 100 mL×1 | $455 | 2-8°C, 24 months |
| CryoStor®CS5 | 07949 | 5% DMSO, serum-free, animal-derived protein-free | 10 mL×5 | $333 | ||
| 07933 | 100 mL×1 Bottle | $465 | ||||
| 07953 | 100 mL ×1 Bag | $595 | ||||
| CryoStor®CS10 | 07959 | 10% DMSO, serum-free, animal-derived protein-free | 10 mL×5 | $375 | ||
| 07952 | 10 mL×16 | $1.200 | ||||
| 07931 | 16 mL×5 | $466 | ||||
| 07930 | 100 mL×1 Bottle | $480 | ||||
| 07955 | 100 mL ×1 Bag | $600 | ||||
| 07940 | 1000 mL ×1 Bag | $6.000 | ||||
| Pricella | Freezing Medium (Serum-free & animal origin-free) | PB180438 | 10% DMSO, with added sedimentation stabilizer and cryoprotectant; serum-free and animal-derived protein-free | 10 mL×5 | $68 | 2-8°C, 12 months |
| 50 mL×1 | $65 | |||||
| 100 mL×1 | $118 |
| Freezing Media | Patient-derived cell cultures 1x106 | SW1 mouse cell line 9x106 | Patient-derived xenograft cells 3x106 |
| 90% FBS + 10% DMSO | 0,7x106 | 7,08x106 | 1,8x106 |
| PRICELLA no serum | 0,65x106 | 6,96x106 | 1,5x106 |
We compared Pricella freezing medium against our standard freezing medium (90% Fetal Calf Serum, 10% Dimethyl Sulfoxide) across three distinct melanoma cell types: patient-derived cell cultures, xenograft cells, and a mouse cell line. Upon thawing, the viability and recovery rates demonstrated comparable efficacy between the two freezing media, indicating overlapping results across all tested cells.
Dr Luisa Lanfrancone
Unit Director- Modeling and Targeting Metastatic Cancer
