Low Endotoxin South American Origin
KEY FEATURES:
SKU Description List Price Promo
RM10432-500ML
Fetal Bovine Serum (FBS) Low Endotoxin South American Origin
319,00€
259,00€
CELL CULTURE MEDIUM & AUXILIARY REAGENTS PROMO!
CLASSICAL MEDIA
SKU
Description
Volume
Price (1–9 Bottles)
Price (>10 Bottles)
PMSTVMSV-500mL
Dulbecco’s Modified Eagle Medium (DMEM), High glucose w/ 4.5gms Glucose per litre, L-Glutamine, 3.7 gms per litre Sodium bicarbonate and Sodium pyruvate
500mL
10,00 €
4,80 €
PMSTVMSH-500mL
Dulbecco’s Modified Eagle Medium (DMEM), High glucose w/ 4.5gms Glucose per litre, Sodium bicarbonate and Sodium pyruvate w/o L-Glutamine
500mL
15,00 €
4,80 €
PMSTVSSV-500mL
RPMI-1640 w/ L-Glutamine and Sodium bicarbonate
500mL
10,00 €
4,80 €
PMSTVSST-500mL
RPMI-1640 w/ Sodium bicarbonate w/o L-Glutamine
500mL
15,00 €
5,10 €
PMSTVESH-500mL
Minimum Essential Medium Eagle, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
500mL
15,00 €
8,70 €
TL1006-500mL
Dulbecco's Phosphate Buffered Saline 1X w/o Phenol red, Calcium and Magnesium
500mL
10,00 €
4,60 €
TL1022-500mL
Dulbecco's Phosphate Buffered Saline 10X w/o Phenol red, Calcium and Magnesium
500mL
40,00 €
6,50 €
PBSAVEMV-100mL
L-glutamine 200 mM, solution, sterile-filtered, suitable for cell culture
100mL
15,00 €
6,60 €
PBSAVSMV-125mL
Antibiotic Solution 100X Liquid w/ 10,000 U Penicillin and 10mg Streptomycin per ml in 0.9% normal saline
125mL
15,00 €
7,10 €
PBSAVMMK-100mL
0.25% Trypsin Solution (with EDTA, phenol red, dissolved in D-Hank's)
100mL
15,00 €
7,10 €
PBSAVMMR-100mL
0.25% Trypsin Solution (with EDTA, dissolved in D-Hank's)
100mL
15,00 €
7,10 €
PBSAVSMR-10mL
250 μg/mL Amphotericin B Solution
10mL
30,00 €
15,20 €
PBSAVEME-100mL
MEM Non-Essential Amino Acid Solution (NEAA) (100x)
100mL
20,00 €
13,90 €
FBS Microgem achieved optimal results in all tests
The tests shown were carried out at the cellular and Preclinical Modelling laboratories of IFOM in Milan.
GROWTH AND VITALITY TEST
U2OS CELL LINE
A-549 CELL LINE
GROWTH AND HYPERACTIVATION TEST
IMR-90 NORMAL FIBROBLAST CELL LINE
A-549 CELL LINE
PLATING EFFICIENCY (COLONY ASSAY)
IMR-90 NORMAL FIBROBLAST CELL LINE
L-929 CELL LINE
Serum
Average Colony Area
N. of Colonies
Relative Average Colony Area
Relative Count of Colony Area 2
L929_1
514,6
223
110,60%
63,20%
L929_2
533,6
418
114,70%
118,40%
L929_3
397,7
199
85,50%
56,40%
L929_4
398,2
179
85,60%
50,70%
L929_5
374,9
157
80,60%
44,50%
L929_6
305,4
274
65,60%
77,60%
L929_7
497
331
106,80%
93,80%
L929_8
293,6
327
63,10%
92,60%
L929_9
465,3
353
100,00%
100,00%
U2OS CELL LINE
L-929 CELL LINE
Serum
Average Colony Area
N. of Colonies
Relative Average Colony Area
Relative Count of Colony Area 2
U2OS_1
523,1
1248
103,30%
153,69%
U2OS_2
546,8
1093
108,00%
134,61%
U2OS_3
572
1079
113,00%
132,88%
U2OS_4
273,4
336
54,00%
41,38%
U2OS_5
784,5
1100
155,00%
135,47%
U2OS_6
373,2
638
73,70%
78,57%
U2OS_7
577,6
1111
114,10%
136,82%
U2OS_8
397,7
835
78,60%
102,83%
U2OS_9
506,2
812
100,00%
100,00%
INFECTION TEST
For the infection test, only the three best candidates were tested, chosen by evaluating the results of the growth curve and colony assay
% of GFP POSITIVE CELLS
Dilution
Siero 1
Siero 2
Siero 7
10-2
62,50%
48%
68,10%
10-3
18,50%
10,70%
21,50%
10-4
2,30%
1,30%
2,60%
Titolo virale 1,94 × 107 TU/mL
9,9 × 106 TU/mL
2,26 × 107 TU/mL
GROWTH AND HYPERACTIVATION TEST
IMR-90 NORMAL FIBROBLAST CELL LINE
PLATING EFFICIENCY (COLONY ASSAY)
L-929 CELL LINE
Serum
Average Colony Area
N. of Colonies
Relative Average Colony Area
Relative Count of Colony Area 2
L929_1
514,6
223
110,60%
63,20%
L929_2
533,6
418
114,70%
118,40%
L929_3
397,7
199
85,50%
56,40%
L929_4
398,2
179
85,60%
50,70%
L929_5
374,9
157
80,60%
44,50%
L929_6
305,4
274
65,60%
77,60%
L929_7
497
331
106,80%
93,80%
L929_8
293,6
327
63,10%
92,60%
L929_9
465,3
353
100,00%
100,00%
