Servizi

Panel Design Services

Elabscience fornisce ai propri clienti un servizio professionale e gratuito di progettazione dei pannelli.

È sufficiente fornire al nostro supporto tecnico le informazioni relative ai marker del vostro esperimento di citometria a flusso (specificità, relazioni logiche o riferimenti, livelli di espressione dei marker target) e le informazioni di base sul citometro utilizzato (laser, canali di rilevazione e filtri).

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Caratteristiche del servizio di Panel Design

Personalizzazione

Progettazione su misura in base al tuo esperimento, per garantire che il pannello sia pienamente coerente con i requisiti sperimentali e con la configurazione dello strumento utilizzato.

Professionalità

Un supporto tecnico esperto in citometria a flusso mette a disposizione soluzioni di progettazione del pannello di elevata qualità.

Praticità

Permette di risparmiare tempo ed energie, consentendo di concentrarsi maggiormente sulle attività sperimentali e sul lavoro correlato.

Basic Information
Provided
Select the
Target Markers
Check the Flow
Cytometry Information
Check Fluorochrome
Information
Pair Antigens with
Fluorochromes
Provide the
Panel Design
Elabscience Techsupport Design The Panel

Related services

Elabscience® offers more than 3,000 high-quality Flow Cytometry Antibodies with a variety of dyes and a wide range of intracellular and extracellular markers for your selection, meeting the requirements of your flow cytometry experiment panel design.

Elabscience® can provide you with Labeling Services such as different fluorochrome labeling, biotin labeling, and HRP labeling.

Cases of Panel Design

Detection of T/B/NK (4-color) in human peripheral blood

Panel Design

Purpose Sample Antibody Collocation
Adjust the voltage 1 Blank
Adjust compensation 2 CD45-PerCP
3 CD3-FITC
4 CD16-PE / CD56-PE
5 CD19-APC
PE-FMO in combination with Isotype Control for auxiliary gating 6 CD3-PerCP/Cyanine5.5, CD4-FITC, IL-17A- PE
Full panel 7 CD45-PerCP, CD3-FITC, CD19-APC, Mouse IgG1, k Isotype Control-PE

Tips

  1. This panel has obvious cell populations, and compensation regulation can be performed without single positive tube. But for beginners of Flow Cytometry, it is recommended to set up a single positive tube to adjust compensation.
  2. The detection standard for NK cells is CD3-CD16+CD56+.
  3. In this panel, it is recommended to set Isotype Control for CD16 and CD56, while other indicators can be omitted due to obvious populations.
  4. It is recommended to stain human peripheral blood samples with CD45, which is beneficial for the lymphocyte phylum gating through CD45 and SSC. It is recommended to use the single positive tube of CD45 to start the machine at low speed and set the threshold.
Detection of T/B/NK (4-color) in human peripheral blood

Panel Design

Purpose Sample Antibody Collocation
Adjust the voltage 1 Blank
Adjust compensation 2 CD4-FITC
3 CD25-APCC
4 Foxp3-PE
APC-FMO in combination with Isotype Control for auxiliary gating 5 CD4-FITC, Foxp3-PE, Rat IgG1, k Isotype Control-APC
PE-FMO in combination with Isotype Control for auxiliary gating 6 CD3-PerCP/Cyanine5.5, CD4-FITC, IL-17A- PE
Full panel 7 CD4-FITC, CD25-APC, Foxp3-PE

Tips

  1. Mouse Treg marker is CD4+ CD25+ Foxp3+.
  2. CD4 cell population is obvious, and there is no need of Isotype Control. But CD25 and Foxp3 populations are not obvious, and Isotype Controls are needed.
  3. There is fluorescence spillover, so it is necessary to set single positive tubes for compensation.
  4. Inappropriate use of Fixation/Permeabilization buffer may cause high background and unclear cell clustering. Please be careful.
Detection of Th17 (3-color) in human peripheral blood PBMC

Panel Design

Purpose Sample Antibody Collocation
Adjust the voltage 1 Blank
Adjust compensation 2 CD3-PerCP/Cynanine5.5
3 CD4-FITC
4 IL-17A PE
PE-FMO in combination with Isotype Control for auxiliary gating 5 CD3-PerCP / Cynanine5.5, CD4-FITC, Rat IgG1, k Isotype Control-PE
Full panel 6 CD3-PerCP/Cyanine5.5, CD4-FITC, IL-17A- PE

Tips

  1. After PBMC sorting, it is necessary to first use cytokine stimulating and blocking agents for stimulating and blocking culture (The reagents used in this experiment are: Cytokine Activation and Protein Blocking Kit (E-CK-A091). The cultivation conditions are as follows: after 1 hour of stimulation with a stimulating agent, add a blocking agent and incubate for another 4.5 hours), then collect cells for subsequent Flow Cytometry experiments.
  2. PMA stimulation can cause partial endocytosis of CD4 on the surface of human T cells, so we need to choose the CD4 clone SK3 with minimal impact on endocytosis.
  3. Isotype control for IL-17A is necessary, since the expression of cytokines is generally not high.
  4. CD3+CD4+ IL-17A+ is Th17 type.
  5. The Permeabilization buffer may cause significant damage to cells, so it is recommended that the cell precipitates formed after centrifugation be dispersed into cell suspensions before adding the Permeabilization buffer to reduce cell damage.

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We will provide professional and free Panel Design Services for your experiment.

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