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EnkiLife TSA

Multiple Fluorescent Staining Kit

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EnkiLife TSA - Multiple Fluorescent Staining Kit

EnkiLife TSA Multiple Fluorescent Staining Kit is optimized for multiplex immunofluorescence (IF) on formalin-fixed paraffin-embedded (FFPE) sections, frozen sections, cultured cells (adherent or smear preparations), and organoid models. The workflow is powered by tyramide signal amplification (TSA). Horseradish peroxidase (HRP) catalyzes the conversion of H₂O₂ into highly reactive oxygen radicals that covalently couple fluorophore-conjugated tyramides to electron-rich amino-acid residues (Trp, His, Tyr) in the immediate vicinity of the antigen-antibody complex. Each tyramide is labeled with a spectrally distinct, photostable fluorophore, enabling iterative cycles of antibody binding, HRP-mediated amplification, and fluorophore deposition.

Product Advantages

7-Plex Signals from a Single Slide

7 spectrally resolved TSA fluorophores (480-750 nm) for any experimental panel

Flexible Antibody Selection

Compatible with primary antibodies from rabbit and mouse

Broad Applications

Works with FFPE sections, frozen sections, cells, and organoids

All-in-One Components

Complete kit with tyramides, buffers, reagents, and mounting medium

Gentle Antibody Elution

Special buffer removes antibodies while preserving antigens and tissue integrity

25% PROMO
SKU Description List Price Promo
RA10008-20T 3-Color Multiple Fluorescent Staining Kit (mIHC) - Size: 20T 330,00 € 247,50 €
RA10008-50T 3-Color Multiple Fluorescent Staining Kit (mIHC) - Size: 50T 660,00 € 495,00 €
RA10008-100T 3-Color Multiple Fluorescent Staining Kit (mIHC) - Size: 100T 900,00 € 675,00 €
RA10009-20T 4-Color Multiple Fluorescent Staining Kit (mIHC) - Size: 20T 380,00 € 285,00 €
RA10009-50T 4-Color Multiple Fluorescent Staining Kit (mIHC) - Size: 50T 780,00 € 585,00 €
RA10009-100T 4-Color Multiple Fluorescent Staining Kit (mIHC) - Size: 100T 1.160,00 € 870,00 €
RA10010-20T 5-Color Multiple Fluorescent Staining Kit (mIHC) - Size: 20T 540,00 € 405,00 €
RA10010-50T 5-Color Multiple Fluorescent Staining Kit (mIHC) - Size: 50T 1.080,00 € 810,00 €
RA10010-100T 5-Color Multiple Fluorescent Staining Kit (mIHC) - Size: 100T 1.860,00 € 1.395,00 €
RA10011-20T 6-Color Multiple Fluorescent Staining Kit (mIHC) - Size: 20T 720,00 € 540,00 €
RA10011-50T 6-Color Multiple Fluorescent Staining Kit (mIHC) - Size: 50T 1.430,00 € 1.072,50 €
RA10011-100T 6-Color Multiple Fluorescent Staining Kit (mIHC) - Size: 100T 2.400,00 € 1.800,00 €
RA10012-20T 7-Color Multiple Fluorescent Staining Kit (mIHC) - Size: 20T 980,00 € 735,00 €
RA10012-50T 7-Color Multiple Fluorescent Staining Kit (mIHC) - Size: 50T 1.960,00 € 1.470,00 €
RA10012-100T 7-Color Multiple Fluorescent Staining Kit (mIHC) - Size: 100T 3.280,00 € 2.460,00 €
7-Color Multiple Fluorescent Staining Kit (mIHC)
Components and Dilutions
Components TSA-CY3 TSA-FITC TSA-680 TSA-430 TSA-594 TSA-CY7 TSA diluent 3% H₂O₂
Dilutions 1:200 1:200 1:200 1:200 1:200 1:200 Ready-to-use Ready-to-use
Components Blocking solution Primary antibody diluent Antibody elution reagent HRP-goat anti-rabbit/mouse IgG DAPI staining Anti-fluorescence fading mounting medium
Dilutions Ready-to-use Ready-to-use Ready-to-use Ready-to-use Ready-to-use Ready-to-use
Illustrative Images
Test Sample: Rat kidney

AQP4

Positive staining is evident in principal cells of the inner-medullary collecting ducts as well as in cortical and outer-medullary collecting ducts.

CD31

Widely used for labeling vascular endothelium; expressed in glomerular capillary endothelium and vessels at all hierarchical levels.

TGFβ1

Expression is markedly up-regulated in hypertension and fibrosis models; detected in glomerular e pithelial cells (primarily podocytes) and interstitial fibroblasts.

Zo-1

As a scaffolding protein of tight junctions, it preserves the glomerular filtration barrier and tubular epithelial polarity.

Collagen I

In fibrotic or sclerotic models, it extensively accumulates in the interstitium and glomeruli.

Target Fluorescein Ex (nm) Em (nm) Filters Image Color
AQP4 TSA-430 430nm 480nm Aqua channel Yellow
CD31 TSA-FITC 490nm 520nm FITC channel Green
TGFβ1 TSA-CY3 550nm 570nm CY3 channel Red
Zo-1 TSA-594 590nm 620nm Red channel Cyan
Collagen I TSA-680 680nm 700nm CY5/CY5.5 channel Purple
CASE STUDY
Customer Testimonial
CD19/DAPI
CD20/DAPI
Merge

The test was performed on a patient sample. Lung tissue. Two anti-mouse antibodies were used using TSA Multiple Fluorescent Staining technology. The signal was specific and clear.

Dott.ssa Federica Pisati
Publication Product Recommendation

3-Color Multiple Fluorescent Staining Kit (mIHC)

Codice:
RA10008

Published Journal:

FASEB Journal (WILEY)
DOI: 10.1096/fj.202504507RR
Article Title

NME3 Interacts With NAA10 to Promote RUNX2 Nuclear Translocation and Odontogenic Differentiation in Human Dental Pulp Stem Cells.

Authors

Changxiu Fan¹,² | Ke Xu³

Customer Institution

1Department of Stomatology, Huashan Hospital, Fudan University, Shanghai, China | 2Department of Stomatology, The Fifth People’s Hospital of Shanghai, Fudan University, Shanghai, China | 3Department of Spinal Surgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, China

Applied Experiment

mIHC

CITED PRODUCT
RA10008 - 3-Color Multiple Fluorescent Staining Kit (mIHC)
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